Recombinant protein expression in microbial systems

Recombinant protein expression in microbial systems

INTRODUCTION The emergence of recombinant DNA technology during the early 70’s set a revolution in molecular biology. This set of techniques was strengthened even further later on with the introduction of the polymerase chain reaction and allowed scientists to explore and understand essential life processes in an easy and straightforward way. It also marked the birth of the modern biotech indus...

Advances and challenges in recombinant protein expression in Escherichia coli

Characterization of protein expression in recombinant systems

BioTechniques 381 Characterization of protein expression in recombinant systems has been enhanced through the availability of techniques such as Western blot analysis and enzyme-linked immunosorbent assay (ELISA) (1). A limitation of these techniques is the need for an antibody specific to the protein of interest. The development of epitope tags such as c-myc and HA has addressed this limitatio...

Gene Expression Systems and Recombinant Protein Purification

There is no substance as important as deoxyribonucleic acid as it carries within its own structure the hereditary in formations that determines the structure of proteins, the important molecule of life. Deoxyribonucleic acid transcribe messenger ribonucleic acid which finally translates protein the essential macromolecules. Through gene expression the information contained in gene can be utiliz...

Expression of Recombinant Protein B Subunit Pili from Vibrio Cholera

Background & Aims: Vibrio cholerae is a gram-negative bacterial pathogen that causes cholera disease. Following ingestion by a host and entry into the upper intestine, V. cholera colonizes and begins to emit enterotoxin. One of the most pathogenic factors of Vibrio cholera is toxin-coregulated pili (TCP). ToxinCoregulated pili is as the primary factor requiered for the colonization and insisten...